Chapter title |
Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells
|
---|---|
Chapter number | 15 |
Book title |
Flow Cytometry Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7346-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7344-6, 978-1-4939-7346-0
|
Authors |
Maik Herbig, Martin Kräter, Katarzyna Plak, Paul Müller, Jochen Guck, Oliver Otto |
Abstract |
Real-time deformability cytometry (RT-DC) is a microfluidic technique that allows to capture and evaluate morphology and rheology of up to 1000 cells/s in a constricted channel. The cells are deformed without mechanical contact by hydrodynamic forces and are quantified in real-time without the need of additional handling or staining procedures. Segmented pictures of the cells are stored and can be used for further analysis. RT-DC is sensitive to alterations of the cytoskeleton, which allows, e.g., to show differences in cell cycle phases, identify different subpopulations in whole blood and to study mechanical stiffening of cells entering a dormant state. The abundance of the obtainable parameters and the interpretation as mechanical readout is an analytical challenge that needs standardization. Here, we will provide guidelines for measuring and post-processing of RT-DC data. |
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Demographic breakdown
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Unknown | 17 | 28% |
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