↓ Skip to main content
What is this page? Embed badge

Plant Membrane Proteomics

Overview of attention for book
Cover of 'Plant Membrane Proteomics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments Prior to Proteomic Analysis
  3. Altmetric Badge
    Chapter 2 Isolation and Purity Assessment of Membranes from Norway Spruce
  4. Altmetric Badge
    Chapter 3 Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis
  5. Altmetric Badge
    Chapter 4 Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis
  6. Altmetric Badge
    Chapter 5 Isolation, Purity Assessment, and Proteomic Analysis of Nuclei
  7. Altmetric Badge
    Chapter 6 Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient
  8. Altmetric Badge
    Chapter 7 Analyzing the Vacuolar Membrane (Tonoplast) Proteome
  9. Altmetric Badge
    Chapter 8 Preparation of Membrane Fractions (Envelope, Thylakoids, Grana, and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies
  10. Altmetric Badge
    Chapter 9 Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria
  11. Altmetric Badge
    Chapter 10 Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development
  12. Altmetric Badge
    Chapter 11 Sample Preparation for Analysis of the Plant Mitochondrial Membrane Proteome
  13. Altmetric Badge
    Chapter 12 Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS
  14. Altmetric Badge
    Chapter 13 Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis
  15. Altmetric Badge
    Chapter 14 Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses
  16. Altmetric Badge
    Chapter 15 Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters
  17. Altmetric Badge
    Chapter 16 MSE for Label-Free Absolute Protein Quantification in Complex Proteomes
  18. Altmetric Badge
    Chapter 17 Identification and Characterization of Plant Membrane Proteins Using ARAMEMNON
  19. Altmetric Badge
    Chapter 18 VANTED: A Tool for Integrative Visualization and Analysis of -Omics Data
Attention for Chapter 3: Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
10 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis
Chapter number 3
Book title
Plant Membrane Proteomics
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7411-5_3
Pubmed ID
29086395
Book ISBNs
978-1-4939-7409-2, 978-1-4939-7411-5
Authors

Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty

Abstract

Proteome profiling aims to unravel the mystery of biological complexity encoded by the genome. The successful proteome profiling largely depends upon analytical approaches because single-step proteome characterization of eukaryotic cells is difficult due to the large number of proteins expressed and their complex physiochemical properties. Organellar proteomics helps in identifying a refined set of proteins by pinpointing certain activities to specific organelles, thereby increasing our knowledge of cellular processes. The reliability of a plant organelle proteome is intimately dependent on the purity of the organelle preparation. Methodological improvements in sample handling, organelle fractionation, and protein extraction are therefore crucial to plant subcellular proteomics. The nuclear proteins are organized into complex regulatory networks and perform varied cellular functions. Therefore, characterization of the nuclear proteome is an important step toward accumulating knowledge about regulation of gene expression and function. In this chapter, we present methods for the isolation of nuclei, purification of nuclear proteins, and proteome profiling that have been adapted for proteomic characterization of economically important crop species, such as chickpea.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 20%
Other 1 10%
Student > Ph. D. Student 1 10%
Unknown 6 60%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 10%
Materials Science 1 10%
Engineering 1 10%
Unknown 7 70%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2017.
All research outputs
#18,575,277
of 23,007,053 outputs
Outputs from Methods in molecular biology
#7,962
of 13,159 outputs
Outputs of similar age
#330,463
of 442,275 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,498 outputs
Altmetric has tracked 23,007,053 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,159 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,275 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.