↓ Skip to main content

Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules

Overview of attention for article published in Analytical Biochemistry, June 2014
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

blogs
1 blog

Citations

dimensions_citation
15 Dimensions

Readers on

mendeley
24 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules
Published in
Analytical Biochemistry, June 2014
DOI 10.1016/j.ab.2014.05.023
Pubmed ID
Authors

Minh-Dao Duong-Thi, Gunnar Bergström, Carl-Fredrik Mandenius, Maria Bergström, Tomas Fex, Sten Ohlson

Abstract

In this study, we compared affinity data from surface plasmon resonance (SPR) and weak affinity chromatography (WAC), two established techniques for determination of weak affinity (mM-μM) small molecule-protein interactions. In the current comparison, thrombin was used as target protein. In WAC the affinity constant (KD) was determined from retention times, and in SPR it was determined by Langmuir isotherm fitting of steady-state responses. Results indicate a strong correlation between the two methods (R(2)=0.995, P<0.0001).

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 4%
Czechia 1 4%
Unknown 22 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 33%
Student > Ph. D. Student 6 25%
Student > Master 2 8%
Professor 1 4%
Other 1 4%
Other 2 8%
Unknown 4 17%
Readers by discipline Count As %
Chemistry 7 29%
Agricultural and Biological Sciences 7 29%
Biochemistry, Genetics and Molecular Biology 3 13%
Philosophy 1 4%
Medicine and Dentistry 1 4%
Other 1 4%
Unknown 4 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2014.
All research outputs
#4,807,143
of 25,371,288 outputs
Outputs from Analytical Biochemistry
#1,061
of 8,533 outputs
Outputs of similar age
#44,471
of 243,252 outputs
Outputs of similar age from Analytical Biochemistry
#3
of 28 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,533 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,252 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.