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Mendeley readers
| Chapter title |
Kidney Research
|
|---|---|
| Chapter number | 2 |
| Book title |
Kidney Research
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3353-2_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3351-8, 978-1-4939-3353-2
|
| Authors |
Tan, Sven-Jean, Hewitson, Tim D, Hewitson, Tim D., Sven-Jean Tan, Tim D. Hewitson |
| Abstract |
The renal fibroblast and phenotypically related myofibroblast are universally present in all forms of progressive kidney disease. The in vitro study of the fibroblast, its behavior, and factors affecting its activity is therefore key to understanding both its role and significance. In this protocol, we describe a reproducible method for selective propagation and culture of primary human renal fibroblasts from the human kidney cortex. Techniques for their isolation, subculture, characterization, and cryogenic storage and retrieval are described in detail. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 10% |
| Australia | 1 | 10% |
| Unknown | 8 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 40% |
| Student > Bachelor | 2 | 20% |
| Librarian | 1 | 10% |
| Professor | 1 | 10% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 30% |
| Agricultural and Biological Sciences | 2 | 20% |
| Veterinary Science and Veterinary Medicine | 1 | 10% |
| Physics and Astronomy | 1 | 10% |
| Unknown | 3 | 30% |