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Development of a new tissue injector for subretinal transplantation of human embryonic stem cell derived retinal pigmented epithelium

Overview of attention for article published in International Journal of Retina and Vitreous , October 2017
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Title
Development of a new tissue injector for subretinal transplantation of human embryonic stem cell derived retinal pigmented epithelium
Published in
International Journal of Retina and Vitreous , October 2017
DOI 10.1186/s40942-017-0095-6
Pubmed ID
Authors

Rodrigo A. Brant Fernandes, Francisco R. Stefanini, Paulo Falabella, Michael J. Koss, Trent Wells, Bruno Diniz, Ramiro Ribeiro, Paulo Schor, Mauricio Maia, Fernando M. Penha, David R. Hinton, Yu-Chong Tai, Mark Humayun

Abstract

Subretinal cell transplantation is a challenging surgical maneuver. This paper describes the preliminary findings of a new tissue injector for subretinal implantation of an ultrathin non-absorbable substrate seeded with human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE). Ultrathin Parylene-C substrates measuring 3.5 mm × 6.0 mm seeded with hESC-RPE (implant referred to as CPCB-RPE1) were implanted into the subretinal space of 12 Yucatan minipigs. Animals were euthanized immediately after the procedure and underwent spectral domain optical coherence tomography (SD-OCT) and histological analysis to assess the subretinal placement of the implant. Evaluation of the hESC-RPE cells seeded on the substrate was carried out before and after implantation using standard cell counting techniques. The tissue injector delivered the CPCB-RPE1 implant through a 1.5 mm sclerotomy and a 1.0-1.5 mm retinectomy. SD-OCT scans and histological examination revealed that substrates were precisely placed in the subretinal space, and that the hESC-RPE cell monolayer continued to cover the surface of the substrate after the surgical procedure. This innovative tissue injector was able to efficiently deliver the implant in the subretinal space of Yucatan minipigs, preventing significant hESC-RPE cell loss, minimizing tissue trauma, surgical complications and postoperative inflammation.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 21%
Professor > Associate Professor 2 14%
Other 2 14%
Professor 2 14%
Student > Ph. D. Student 2 14%
Other 3 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 21%
Unspecified 3 21%
Medicine and Dentistry 3 21%
Pharmacology, Toxicology and Pharmaceutical Science 2 14%
Chemistry 1 7%
Other 2 14%