| Chapter title |
Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage
|
|---|---|
| Chapter number | 8 |
| Book title |
Bacteriophage Therapy
|
| Published in |
Methods in molecular biology, November 2017
|
| DOI | 10.1007/978-1-4939-7395-8_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7394-1, 978-1-4939-7395-8
|
| Authors |
Libera Latino, Christine Pourcel, Latino, Libera, Pourcel, Christine |
| Abstract |
Bacteria and bacteriophages coexist and coevolve, bacteriophages being obligatory predators exerting an evolutionary pressure on their prey. Mechanisms in action vary depending on the bacterial genomic content and on the regulation of the bacteriophage cycle. To assess the multiplicity of bacterial genes involved in resistance as well as the changes in the bacteriophage interactions with the bacteria, it is necessary to isolate and investigate large numbers of independent resistant variants. Here we describe protocols that have been applied to the study of Pseudomonas aeruginosa and four of its virulent bacteriophages belonging to the Podoviridae and Myoviridae bacteriophage families. Mutations are identified using whole genome sequencing of resistant variants. Phenotypic analyses are performed to describe the changes conferred by the mutations. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 22% |
| Other | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Professor | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Other | 2 | 22% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 33% |
| Agricultural and Biological Sciences | 2 | 22% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
| Computer Science | 1 | 11% |
| Immunology and Microbiology | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |