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The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C

Overview of attention for article published in Nature, November 2014
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Average Attention Score compared to outputs of the same age and source

Citations

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205 Mendeley
Title
The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C
Published in
Nature, November 2014
DOI 10.1038/nature13911
Pubmed ID
Authors

Daisuke Izawa, Jonathon Pines

Abstract

The spindle assembly checkpoint (SAC) maintains genomic stability by delaying chromosome segregation until the last chromosome has attached to the mitotic spindle. The SAC prevents the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase from recognizing cyclin B and securin by catalysing the incorporation of the APC/C co-activator, CDC20, into a complex called the mitotic checkpoint complex (MCC). The SAC works through unattached kinetochores generating a diffusible 'wait anaphase' signal that inhibits the APC/C in the cytoplasm, but the nature of this signal remains a key unsolved problem. Moreover, the SAC and the APC/C are highly responsive to each other: the APC/C quickly targets cyclin B and securin once all the chromosomes attach in metaphase, but is rapidly inhibited should kinetochore attachment be perturbed. How this is achieved is also unknown. Here, we show that the MCC can inhibit a second CDC20 that has already bound and activated the APC/C. We show how the MCC inhibits active APC/C and that this is essential for the SAC. Moreover, this mechanism can prevent anaphase in the absence of kinetochore signalling. Thus, we propose that the diffusible 'wait anaphase' signal could be the MCC itself, and explain how reactivating the SAC can rapidly inhibit active APC/C.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 205 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 4 2%
United States 3 1%
France 2 <1%
Germany 1 <1%
Belgium 1 <1%
Netherlands 1 <1%
Austria 1 <1%
Unknown 192 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 70 34%
Researcher 41 20%
Student > Bachelor 30 15%
Student > Master 23 11%
Professor > Associate Professor 9 4%
Other 32 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 113 55%
Biochemistry, Genetics and Molecular Biology 55 27%
Medicine and Dentistry 12 6%
Unspecified 11 5%
Chemistry 6 3%
Other 8 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2015.
All research outputs
#994,503
of 12,680,451 outputs
Outputs from Nature
#27,501
of 65,621 outputs
Outputs of similar age
#21,764
of 229,237 outputs
Outputs of similar age from Nature
#634
of 923 outputs
Altmetric has tracked 12,680,451 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 65,621 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 73.7. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 229,237 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 923 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.