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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
|
|---|---|
| Published in |
Genome Biology, November 2017
|
| DOI | 10.1186/s13059-017-1355-3 |
| Pubmed ID | |
| Authors |
Sojung Kim, Taegeun Bae, Jaewoong Hwang, Jin-Soo Kim |
| Abstract |
We report that engineered Cas9 variants with improved specificity-eCas9-1.1 and Cas9-HF1-are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5' terminus, relative to target DNA sequences. Because the nucleotide at the 5' end of sgRNAs, expressed under the control of the commonly-used U6 promoter, is fixed to a guanine, these attenuated Cas9 variants are not useful at many target sites. By using sgRNAs with matched 5' nucleotides, produced by linking them to a self-cleaving ribozyme, the editing activity of Cas9 variants can be rescued without sacrificing high specificity. |
X Demographics
The data shown below were collected from the profiles of 16 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 6% |
| United States | 1 | 6% |
| Canada | 1 | 6% |
| Russia | 1 | 6% |
| Netherlands | 1 | 6% |
| Saudi Arabia | 1 | 6% |
| India | 1 | 6% |
| United Kingdom | 1 | 6% |
| Unknown | 8 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 63% |
| Scientists | 4 | 25% |
| Science communicators (journalists, bloggers, editors) | 2 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 122 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 122 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 33 | 27% |
| Researcher | 32 | 26% |
| Student > Master | 12 | 10% |
| Student > Bachelor | 9 | 7% |
| Professor > Associate Professor | 3 | 2% |
| Other | 6 | 5% |
| Unknown | 27 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 46 | 38% |
| Agricultural and Biological Sciences | 30 | 25% |
| Chemistry | 4 | 3% |
| Engineering | 3 | 2% |
| Medicine and Dentistry | 3 | 2% |
| Other | 8 | 7% |
| Unknown | 28 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2024.
All research outputs
#2,031,250
of 25,382,440 outputs
Outputs from Genome Biology
#1,718
of 4,468 outputs
Outputs of similar age
#38,774
of 335,891 outputs
Outputs of similar age from Genome Biology
#36
of 59 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,468 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,891 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.