Title |
Repeated intra-articular injection of allogeneic mesenchymal stem cells causes an adverse response compared to autologous cells in the equine model
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Published in |
Stem Cell Research & Therapy, February 2017
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DOI | 10.1186/s13287-017-0503-8 |
Pubmed ID | |
Authors |
Amanda-Jo Joswig, Alexis Mitchell, Kevin J. Cummings, Gwendolyn J. Levine, Carl A. Gregory, Roger Smith, Ashlee E. Watts |
Abstract |
Intra-articular injection of mesenchymal stem cells (MSCs) is efficacious in osteoarthritis therapy. A direct comparison of the response of the synovial joint to intra-articular injection of autologous versus allogeneic MSCs has not been performed. The objective of this study was to assess the clinical response to repeated intra-articular injection of allogeneic versus autologous MSCs prepared in a way to minimize xeno-contaminants in a large animal model. Intra-articular injections of bone marrow-derived, culture-expanded MSCs to a forelimb metacarpophalangeal joint were performed at week 0 and week 4 (six autologous; six autologous with xeno-contamination; six allogeneic). In the week following each injection, clinical and synovial cytology evaluations were performed. Following the first intra-articular injection, there were no differences in clinical parameters over time. Following the second intra-articular injection, there was a significant adverse response of the joint to allogeneic MSCs and autologous MSCs with xeno-contamination with elevated synovial total nucleated cell counts. There was also significantly increased pain from joints injected with autologous MSCs with xeno-contamination. Repeated intra-articular injection of allogeneic MSCs results in an adverse clinical response, suggesting there is immune recognition of allogeneic MSCs upon a second exposure. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 195 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 30 | 15% |
Student > Ph. D. Student | 18 | 9% |
Researcher | 18 | 9% |
Student > Master | 17 | 9% |
Student > Postgraduate | 13 | 7% |
Other | 35 | 18% |
Unknown | 64 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 39 | 20% |
Medicine and Dentistry | 25 | 13% |
Biochemistry, Genetics and Molecular Biology | 23 | 12% |
Agricultural and Biological Sciences | 16 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 6 | 3% |
Other | 18 | 9% |
Unknown | 68 | 35% |