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Cryopreservation at −75 °C of Agaricus subrufescens on wheat grains with sucrose

Overview of attention for article published in Brazilian Journal of Microbiology, April 2018
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Title
Cryopreservation at −75 °C of Agaricus subrufescens on wheat grains with sucrose
Published in
Brazilian Journal of Microbiology, April 2018
DOI 10.1016/j.bjm.2017.08.003
Pubmed ID
Authors

Lienine Luiz Zaghi Júnior, Ana Daniela Lopes, Fábio Aparecido Cordeiro, Itaruã Machri Colla, Míria Benetati Delgado Bertéli, Juliana Silveira do Valle, Giani Andrea Linde, Nelson Barros Colauto

Abstract

Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20°C and at -75°C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75°C or at -20°C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20°C is not effective to keep mycelial viability of this fungus. Cryopreservation at -75°C is effective when sucrose is used in substrates and/or cryotubes. Without sucrose, cryopreservation at -75°C is effective only when wheat grains are used. Physiological characteristic as mycelial colony diameter is negatively affected when potato dextrose agar is used and unaffected when wheat grain is used after two-year cryopreservation at -75°C. The fungus genome does not show alteration after two-year cryopreservation at -75°C.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 25%
Student > Master 3 15%
Student > Doctoral Student 2 10%
Other 1 5%
Student > Ph. D. Student 1 5%
Other 3 15%
Unknown 5 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 25%
Agricultural and Biological Sciences 3 15%
Pharmacology, Toxicology and Pharmaceutical Science 2 10%
Chemical Engineering 1 5%
Environmental Science 1 5%
Other 1 5%
Unknown 7 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2017.
All research outputs
#10,978,531
of 12,355,257 outputs
Outputs from Brazilian Journal of Microbiology
#328
of 363 outputs
Outputs of similar age
#297,819
of 356,365 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#21
of 25 outputs
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So far Altmetric has tracked 363 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.