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Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells

Overview of attention for article published in BMC Genomics, November 2014
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Title
Simulation and estimation of gene number in a biological pathway using almost complete saturation mutagenesis screening of haploid mouse cells
Published in
BMC Genomics, November 2014
DOI 10.1186/1471-2164-15-1016
Pubmed ID
Authors

Masahiro Tokunaga, Chikara Kokubu, Yusuke Maeda, Jun Sese, Kyoji Horie, Nakaba Sugimoto, Taroh Kinoshita, Kosuke Yusa, Junji Takeda

Abstract

Genome-wide saturation mutagenesis and subsequent phenotype-driven screening has been central to a comprehensive understanding of complex biological processes in classical model organisms such as flies, nematodes, and plants. The degree of "saturation" (i.e., the fraction of possible target genes identified) has been shown to be a critical parameter in determining all relevant genes involved in a biological function, without prior knowledge of their products. In mammalian model systems, however, the relatively large scale and labor intensity of experiments have hampered the achievement of actual saturation mutagenesis, especially for recessive traits that require biallelic mutations to manifest detectable phenotypes.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 5%
Japan 1 5%
Unknown 17 89%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 37%
Professor > Associate Professor 3 16%
Student > Ph. D. Student 2 11%
Student > Bachelor 2 11%
Student > Master 2 11%
Other 1 5%
Unknown 2 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 63%
Biochemistry, Genetics and Molecular Biology 3 16%
Neuroscience 1 5%
Medicine and Dentistry 1 5%
Unknown 2 11%