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Attention Score in Context
| Title |
Effects of L-Malic Acid on Alpha-Glucosidase: Inhibition Kinetics and Computational Molecular Dynamics Simulations
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, December 2014
|
| DOI | 10.1007/s12010-014-1429-6 |
| Pubmed ID | |
| Authors |
Lin Gou, Yi Zhan, Jinhyuk Lee, Xuan Li, Zhi-Rong Lü, Hai-Meng Zhou, Hang Lu, Xi-Yao Wang, Yong-Doo Park, Jun-Mo Yang |
| Abstract |
The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical non-competitive type, as well as in a fast inactivate process without detectable time course. The tertiary conformation study with an application of spectrofluorimetry showed that MA modulated the tertiary structural conformation of alpha-glucosidase both on the overall and on regional active site pocket, which monitored by red-shift intrinsic fluorescence peak with decreases intensities, and the significant intensity increasing of 1-anilinonaphthalene-8-sulfonate (ANS)-binding fluorescence, respectively. To have more insight, we also adapted the computational molecular dynamics (MD) simulations. The results showed that MA was located in the entrance of active pocket for the catalytic reaction and blocked the passage of substrate. It confirmed that MA inhibits as a non-competitive type, not direct docking to the glucose binding site. Our study provides important molecular mechanisms to figure out alpha-glucosidase inhibition that might associate to development of type 2 diabetes mellitus drug. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 4 | 19% |
| Researcher | 3 | 14% |
| Unspecified | 2 | 10% |
| Student > Master | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Other | 4 | 19% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 24% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 14% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Unspecified | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Other | 3 | 14% |
| Unknown | 4 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 October 2015.
All research outputs
#20,245,139
of 22,772,779 outputs
Outputs from Applied Biochemistry and Biotechnology
#2,031
of 2,500 outputs
Outputs of similar age
#301,396
of 359,774 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#45
of 69 outputs
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So far Altmetric has tracked 2,500 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 69 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.