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First evidence of Zika virus venereal transmission in Aedes aegypti mosquitoes

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, November 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#23 of 1,242)
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
1 tweeter

Citations

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13 Dimensions

Readers on

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110 Mendeley
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Title
First evidence of Zika virus venereal transmission in Aedes aegypti mosquitoes
Published in
Memórias do Instituto Oswaldo Cruz, November 2017
DOI 10.1590/0074-02760170329
Pubmed ID
Authors

Jordam William Pereira-Silva, Valdinete Alves do Nascimento, Heliana Christy Matos Belchior, Jéssica Feijó Almeida, Felipe Arley Costa Pessoa, Felipe Gomes Naveca, Claudia María Ríos-Velásquez

Abstract

Aedes aegypti is considered the main Zika virus (ZIKV) vector, and is thought to be responsible for the 2015-2016 outbreak in Brazil. Zika positive Ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of ZIKV may occur. In this study, we aimed to demonstrate that venereal transmission of ZIKV by Ae. aegypti can occur under laboratory conditions. Ae. aegypti collected in the city of Manaus, confirmed as negative for Zika, Dengue and Chikungunya virus by reverse transcription real-time polymerase chain reaction (RT-qPCR) (AaM3V- strain), were reared under laboratory conditions and used for the experiments. The ZIKV used in this study was isolated from a patient presenting with symptoms; ZIKV was confirmed by RT-qPCR. Experiment 1: virgin male mosquitoes of AaM3V- strain were intrathoracically inoculated with a ZIKV suspension; four days after injection, they were transferred to a cage containing virgin females of AaM3V- strain and left to copulate for five days. Experiment 2: virgin female mosquitoes of AaM3V- strain were orally infected with a ZIKV suspension by blood feeding membrane assay; nine days after blood feeding, they were placed in cages with Ae. aegypti AaM3V- virgin males and left to copulate for four days. After copulation, all mosquitoes were individually evaluated for viral infection by RT-qPCR. The mean infection rate in Experiment 1 and Experiment 2 was 45% and 35%, respectively. In both experiments, cycle threshold values ranged from 13 to 35, indicating the presence of viral genomes. Ae. aegypti males intrathoracically inoculated with a ZIKV suspension are infected and can transmit the virus to uninfected females by mating. Moreover, Ae. aegypti females orally infected with a ZIKV suspension can transmit the virus to uninfected males by copulation. This study shows that ZIKV infection of Ae. aegypti mosquitoes occurs not only during blood feeding, but also during copulation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 110 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 110 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 21 19%
Researcher 18 16%
Student > Bachelor 16 15%
Student > Ph. D. Student 12 11%
Other 8 7%
Other 19 17%
Unknown 16 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 32 29%
Biochemistry, Genetics and Molecular Biology 13 12%
Medicine and Dentistry 11 10%
Immunology and Microbiology 6 5%
Pharmacology, Toxicology and Pharmaceutical Science 5 5%
Other 22 20%
Unknown 21 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2021.
All research outputs
#1,362,674
of 19,233,288 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#23
of 1,242 outputs
Outputs of similar age
#42,973
of 429,440 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#2
of 39 outputs
Altmetric has tracked 19,233,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,242 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 429,440 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.