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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Characterization of Constitutive Promoters for piggyBac Transposon-Mediated Stable Transgene Expression in Mesenchymal Stem Cells (MSCs)
|
|---|---|
| Published in |
PLOS ONE, April 2014
|
| DOI | 10.1371/journal.pone.0094397 |
| Pubmed ID | |
| Authors |
Sheng Wen, Hongmei Zhang, Yasha Li, Ning Wang, Wenwen Zhang, Ke Yang, Ningning Wu, Xian Chen, Fang Deng, Zhan Liao, Junhui Zhang, Qian Zhang, Zhengjian Yan, Wei Liu, Zhonglin Zhang, Jixing Ye, Youlin Deng, Guolin Zhou, Hue H. Luu, Rex C. Haydon, Lewis L. Shi, Tong-Chuan He, Guanghui Wei |
| Abstract |
Multipotent mesenchymal stem cells (MSCs) can undergo self-renewal and give rise to multi-lineages under given differentiation cues. It is frequently desirable to achieve a stable and high level of transgene expression in MSCs in order to elucidate possible molecular mechanisms through which MSC self-renewal and lineage commitment are regulated. Retroviral or lentiviral vector-mediated gene expression in MSCs usually decreases over time. Here, we choose to use the piggyBac transposon system and conduct a systematic comparison of six commonly-used constitutive promoters for their abilities to drive RFP or firefly luciferase expression in somatic HEK-293 cells and MSC iMEF cells. The analyzed promoters include three viral promoters (CMV, CMV-IVS, and SV40), one housekeeping gene promoter (UbC), and two composite promoters of viral and housekeeping gene promoters (hEFH and CAG-hEFH). CMV-derived promoters are shown to drive the highest transgene expression in HEK-293 cells, which is however significantly reduced in MSCs. Conversely, the composite promoter hEFH exhibits the highest transgene expression in MSCs whereas its promoter activity is modest in HEK-293 cells. The reduced transgene expression driven by CMV promoters in MSCs may be at least in part caused by DNA methylation, or to a lesser extent histone deacetlyation. However, the hEFH promoter is not significantly affected by these epigenetic modifications. Taken together, our results demonstrate that the hEFH composite promoter may be an ideal promoter to drive long-term and high level transgene expression using the piggyBac transposon vector in progenitor cells such as MSCs. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Unknown | 52 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 23% |
| Student > Master | 8 | 15% |
| Student > Bachelor | 5 | 9% |
| Researcher | 5 | 9% |
| Professor > Associate Professor | 3 | 6% |
| Other | 8 | 15% |
| Unknown | 12 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 30% |
| Biochemistry, Genetics and Molecular Biology | 11 | 21% |
| Materials Science | 3 | 6% |
| Psychology | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 15 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2014.
All research outputs
#20,246,428
of 22,774,233 outputs
Outputs from PLOS ONE
#173,460
of 194,339 outputs
Outputs of similar age
#194,985
of 228,078 outputs
Outputs of similar age from PLOS ONE
#4,647
of 5,347 outputs
Altmetric has tracked 22,774,233 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 194,339 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 5,347 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.