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Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9

Overview of attention for article published in Parasites & Vectors, December 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (60th percentile)
  • Good Attention Score compared to outputs of the same age and source (65th percentile)

Mentioned by

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6 tweeters

Citations

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6 Dimensions

Readers on

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44 Mendeley
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Title
Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9
Published in
Parasites & Vectors, December 2017
DOI 10.1186/s13071-017-2539-0
Pubmed ID
Authors

Dexuan Kuang, Jichen Qiao, Zhou Li, Weiwei Wang, Hui Xia, Lubin Jiang, Jiejie Dai, Qiang Fang, Xueyu Dai

Abstract

Plasmodium falciparum is the deadliest malaria parasite. Currently, there are seldom commercial antibodies against P. falciparum proteins, which greatly limits the study on Plasmodium. CRISPR/Cas9 is an efficient genome editing method, which has been employed in various organisms. However, the use of this technique in P. falciparum is still limited to gene knockout, site-specific mutation and generation of green fluorescent protein (GFP) reporter line with disruption of inserted sites. We have adapted the CRISPR/Cas9 system to add commercial tag sequences to endogenous genes of P. falciparum. To add HA or HA-TY1 tags to ck2β1, ck2α and stk, pL6cs-hDHFR-ck2β1/ck2α/stk was constructed, which contained sequences of tags, specific homologous arms, and sgRNA. The P. falciparum 3D7 strain was subsequently transfected with pUF1-BSD-Cas9 and pL6cs-hDHFR-ck2β1/ck2α/stk plasmids via electroporation. After that, BSD and WR99210 drugs were added to the culture to screen parasites containing both plasmids. Twenty days after electroporation, live parasites appeared and were collected to check the tagging by PCR, DNA sequencing, Western blotting and immuno-fluorescence assays. The results showed that the tags were successfully integrated into the C-terminus of these three proteins. We have improved the method to integrate tags to Plasmodium falciparum genes using the CRISPR/Cas9 method, which lays the foundation for further study of Plasmodium falciparum at the molecular level.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 32%
Student > Bachelor 7 16%
Student > Master 6 14%
Researcher 6 14%
Student > Doctoral Student 2 5%
Other 3 7%
Unknown 6 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 34%
Immunology and Microbiology 8 18%
Agricultural and Biological Sciences 4 9%
Medicine and Dentistry 3 7%
Pharmacology, Toxicology and Pharmaceutical Science 3 7%
Other 4 9%
Unknown 7 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2018.
All research outputs
#7,120,686
of 13,801,769 outputs
Outputs from Parasites & Vectors
#1,267
of 3,688 outputs
Outputs of similar age
#154,242
of 397,127 outputs
Outputs of similar age from Parasites & Vectors
#189
of 585 outputs
Altmetric has tracked 13,801,769 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,688 research outputs from this source. They receive a mean Attention Score of 4.5. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 397,127 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 585 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.