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Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens

Overview of attention for article published in Immunologic Research, December 2014
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2 tweeters

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24 Mendeley
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Title
Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens
Published in
Immunologic Research, December 2014
DOI 10.1007/s12026-014-8614-0
Pubmed ID
Authors

Milica Popovic, Radivoje Prodanovic, Raluca Ostafe, Stefan Schillberg, Rainer Fischer, Marija Gavrovic-Jankulovic

Abstract

High-throughput characterization of allergens relies often on phage display technique which is subject to the limitations of a prokaryotic expression system. Substituting the phage display platform with a yeast surface display could lead to fast immunological characterization of allergens with complex structures. Our objective was to evaluate the potential of yeast surface display for characterization of plant-derived food allergens. The coding sequence of mature actinidin (Act d 1) was cloned into pCTCON2 surface display vector. Flow cytometry was used to confirm localization of recombinant Act d 1 on the surface of yeast cells using rabbit polyclonal antisera IgG and IgE from sera of kiwifruit-allergic individuals. Immunological (dot blot, immunoblot ELISA and ELISA inhibition), biochemical (enzymatic activity in gel) and biological (basophil activation) characterization of Act d 1 after solubilization from the yeast cell confirmed that recombinant Act d 1 produced on the surface of yeast cell is similar to its natural counterpart isolated from green kiwifruit. Yeast surface display is a potent technique that enables fast immunochemical characterization of allergens in situ without the need for protein purification and offers an alternative that could lead to improvement of standard immunodiagnostic and immunotherapeutic approaches.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 25%
Student > Ph. D. Student 4 17%
Researcher 3 13%
Student > Doctoral Student 2 8%
Professor 2 8%
Other 4 17%
Unknown 3 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 33%
Biochemistry, Genetics and Molecular Biology 7 29%
Nursing and Health Professions 1 4%
Immunology and Microbiology 1 4%
Chemistry 1 4%
Other 0 0%
Unknown 6 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 December 2014.
All research outputs
#10,061,251
of 12,577,568 outputs
Outputs from Immunologic Research
#442
of 632 outputs
Outputs of similar age
#193,947
of 289,103 outputs
Outputs of similar age from Immunologic Research
#21
of 33 outputs
Altmetric has tracked 12,577,568 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 632 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 289,103 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.