Title |
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy
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Published in |
BMC Genomics, October 2014
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DOI | 10.1186/1471-2164-15-847 |
Pubmed ID | |
Authors |
John P Didion, Ryan J Buus, Zohreh Naghashfar, David W Threadgill, Herbert C Morse, Fernando Pardo-Manuel de Villena |
Abstract |
The crisis of Misidentified and contaminated cell lines have plagued the biological research community for decades. Some repositories and journals have heeded calls for mandatory authentication of human cell lines, yet misidentification of mouse cell lines has received little publicity despite their importance in sponsored research. Short tandem repeat (STR) profiling is the standard authentication method, but it may fail to distinguish cell lines derived from the same inbred strain of mice. Additionally, STR profiling does not reveal karyotypic changes that occur in some high-passage lines and may have functional consequences. Single nucleotide polymorphism (SNP) profiling has been suggested as a more accurate and versatile alternative to STR profiling; however, a high-throughput method for SNP-based authentication of mouse cell lines has not been described. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 2% |
Finland | 1 | 2% |
Netherlands | 1 | 2% |
United States | 1 | 2% |
Unknown | 57 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 16 | 26% |
Student > Ph. D. Student | 14 | 23% |
Student > Bachelor | 5 | 8% |
Other | 5 | 8% |
Student > Master | 4 | 7% |
Other | 9 | 15% |
Unknown | 8 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 19 | 31% |
Agricultural and Biological Sciences | 16 | 26% |
Medicine and Dentistry | 4 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
Immunology and Microbiology | 3 | 5% |
Other | 8 | 13% |
Unknown | 8 | 13% |