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Mendeley readers
| Chapter title |
Propagation and Titration of West Nile Virus on Vero Cells
|
|---|---|
| Chapter number | 3 |
| Book title |
West Nile Virus
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3670-0_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3668-7, 978-1-4939-3670-0
|
| Authors |
Alexander J. McAuley, David W. C. Beasley |
| Abstract |
The propagation and titration of viruses are key virological techniques. Unlike other flaviviruses, such as the dengue viruses, West Nile virus (WNV) grows and plaques very efficiently on Vero cells, usually inducing strong cytopathic effect (CPE) and forming clear plaques. Here, we outline the steps for propagating WNV from culture supernatant stocks and homogenized organ/mosquito samples, as well as for determining virus titers in samples by serial-dilution plaque assay using neutral red or crystal violet stains. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 14% |
| Student > Master | 4 | 14% |
| Researcher | 4 | 14% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Doctoral Student | 2 | 7% |
| Other | 3 | 11% |
| Unknown | 7 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 32% |
| Immunology and Microbiology | 5 | 18% |
| Agricultural and Biological Sciences | 3 | 11% |
| Medicine and Dentistry | 2 | 7% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 29% |