Chapter title |
Integration of Comparative Genomics with Genome-Scale Metabolic Modeling to Investigate Strain-Specific Phenotypical Differences
|
---|---|
Chapter number | 7 |
Book title |
Metabolic Network Reconstruction and Modeling
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7528-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7527-3, 978-1-4939-7528-0
|
Authors |
Jonathan Monk, Emanuele Bosi |
Abstract |
Genome-scale metabolic reconstructions are powerful resources that allow translation biological knowledge and genomic information to phenotypical predictions using a number of constraint-based methods. This approach has been applied in recent years to gain deep insights into the cellular phenotype role of the genes at a systems-level, driving the design of targeted experiments and paving the way for knowledge-based synthetic biology.The identification of genetic determinants underlying the variability at the phenotypical level is crucial to understand the evolutionary trajectories of a bacterial species. Recently, genome-scale metabolic models of different strains have been assembled to highlight the intra-species diversity at the metabolic level. The strain-specific metabolic capabilities and auxotrophies can be used to identify factors related to the lifestyle diversity of a bacterial species.In this chapter, we present the computational steps to perform genome-scale metabolic modeling in the context of comparative genomics, and the different challenges related to this task. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 33% |
Student > Master | 8 | 27% |
Student > Ph. D. Student | 2 | 7% |
Student > Doctoral Student | 1 | 3% |
Student > Bachelor | 1 | 3% |
Other | 3 | 10% |
Unknown | 5 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 11 | 37% |
Agricultural and Biological Sciences | 8 | 27% |
Computer Science | 2 | 7% |
Mathematics | 1 | 3% |
Chemical Engineering | 1 | 3% |
Other | 1 | 3% |
Unknown | 6 | 20% |