Title |
Retracted: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana
|
---|---|
Published in |
EMBO Journal, November 1998
|
DOI | 10.1093/emboj/17.22.6739 |
Pubmed ID | |
Authors |
Gianinna Brigneti, Olivier Voinnet, Wan‐Xiang Li, Liang‐Hui Ji, Shou‐Wei Ding, David C. Baulcombe |
Abstract |
Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of PVY and the 2b protein of CMV. The HCPro acts by blocking the maintenance of PTGS in tissues where silencing had already been set, whereas the 2b protein prevents initiation of gene silencing at the growing points of the plants. Combined with previous findings that viruses are both activators and targets of PTGS, these data provide compelling evidence that PTGS represents a natural mechanism for plant protection against viruses. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
United States | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 2% |
Japan | 3 | <1% |
United Kingdom | 3 | <1% |
France | 2 | <1% |
Australia | 2 | <1% |
India | 2 | <1% |
Switzerland | 2 | <1% |
Brazil | 1 | <1% |
South Africa | 1 | <1% |
Other | 3 | <1% |
Unknown | 293 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 83 | 26% |
Student > Ph. D. Student | 78 | 25% |
Student > Master | 28 | 9% |
Professor | 19 | 6% |
Student > Bachelor | 18 | 6% |
Other | 60 | 19% |
Unknown | 31 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 222 | 70% |
Biochemistry, Genetics and Molecular Biology | 31 | 10% |
Neuroscience | 3 | <1% |
Immunology and Microbiology | 2 | <1% |
Business, Management and Accounting | 2 | <1% |
Other | 16 | 5% |
Unknown | 41 | 13% |