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Proteomic identification of cryostress in epididymal spermatozoa

Overview of attention for article published in Journal of Animal Science and Biotechnology, November 2016
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Title
Proteomic identification of cryostress in epididymal spermatozoa
Published in
Journal of Animal Science and Biotechnology, November 2016
DOI 10.1186/s40104-016-0128-2
Pubmed ID
Authors

Sung Jae Yoon, Md Saidur Rahman, Woo Sung Kwon, Do Yeal Ryu, Yoo Jin Park, Myung Geol Pang

Abstract

Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis (2-DE) results using two commercial antibodies. Cryopreservation reduced viability (%), motility (%), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and capacitated spermatozoa, whereas straightness (STR) and the acrosome reaction increased after cryopreservation (P < 0.05). Nine proteins were differentially expressed (two proteins decreased and seven increased) (>3 fold, P < 0.05) before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation, and the guanylate cyclase pathway. The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 25%
Researcher 3 19%
Other 3 19%
Student > Bachelor 1 6%
Student > Doctoral Student 1 6%
Other 4 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 50%
Veterinary Science and Veterinary Medicine 2 13%
Biochemistry, Genetics and Molecular Biology 1 6%
Unspecified 1 6%
Psychology 1 6%
Other 2 13%
Unknown 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2017.
All research outputs
#9,833,531
of 12,312,517 outputs
Outputs from Journal of Animal Science and Biotechnology
#201
of 256 outputs
Outputs of similar age
#247,050
of 345,829 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#35
of 40 outputs
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