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A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, October 2013
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52 Mendeley
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Title
A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area
Published in
Memórias do Instituto Oswaldo Cruz, October 2013
DOI 10.1590/0074-0276130202
Pubmed ID
Authors

Teiliane Rodrigues Carneiro, Regina Helena Saramago Peralta, Marta Cristhiany Cunha Pinheiro, Sara Menezes de Oliveira, José Mauro Peralta, Fernando Schemelzer Moraes Bezerra

Abstract

The aim of this study was to evaluate the efficacy of a polymerase chain reaction (PCR)-based method to detect Schistosoma mansoni DNA in stool samples from individuals living in a low-endemicity area in Brazil. Of the 125 initial stool samples, 80 were ELISA reactive and eggs were identified in 19 of the samples by parasitological examination. For the PCR evaluations, 56 stool samples were selected and divided into five groups. Groups I-IV were scored negative for S. mansoni eggs by parasitological examination. Groups I and II were ELISA reactive, whereas Groups III and IV were ELISA nonreactive. Groups II and III were positive for other intestinal parasites. PCR testing scored eight samples as positive from these four groups. Group V represented the S. mansoni -positive group and it included ELISA-reactive samples that were scored positive for S. mansoni by one or more parasitological examinations (6/19 were positive by Kato-Katz method, 9/17 by saline gradient and 10/13 by Helmintex®). PCR scored 13 of these 19 samples as positive for S. mansoni . We conclude that while none of these methods yielded 100% sensitivity, a combination of techniques should be effective for improving the detection of S. mansoni infection in low-endemicity areas.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 4%
Mali 1 2%
France 1 2%
Unknown 48 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 19%
Student > Ph. D. Student 7 13%
Student > Bachelor 7 13%
Student > Doctoral Student 4 8%
Student > Postgraduate 3 6%
Other 8 15%
Unknown 13 25%
Readers by discipline Count As %
Medicine and Dentistry 12 23%
Agricultural and Biological Sciences 12 23%
Biochemistry, Genetics and Molecular Biology 6 12%
Immunology and Microbiology 4 8%
Psychology 3 6%
Other 2 4%
Unknown 13 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2015.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#1,012
of 1,502 outputs
Outputs of similar age
#138,070
of 220,038 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#13
of 31 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,502 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 220,038 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.