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A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area

Overview of attention for article published in Memórias do Instituto Oswaldo Cruz, December 2013
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1 tweeter

Citations

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14 Dimensions

Readers on

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48 Mendeley
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Title
A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area
Published in
Memórias do Instituto Oswaldo Cruz, December 2013
DOI 10.1590/0074-0276130202
Pubmed ID
Authors

Teiliane Rodrigues Carneiro, Regina Helena Saramago Peralta, Marta Cristhiany Cunha Pinheiro, Sara Menezes de Oliveira, Jose Mauro Peralta, Fernando Schemelzer Moraes Bezerra

Abstract

The aim of this study was to evaluate the efficacy of a polymerase chain reaction (PCR)-based method to detect Schistosoma mansoni DNA in stool samples from individuals living in a low-endemicity area in Brazil. Of the 125 initial stool samples, 80 were ELISA reactive and eggs were identified in 19 of the samples by parasitological examination. For the PCR evaluations, 56 stool samples were selected and divided into five groups. Groups I-IV were scored negative for S. mansoni eggs by parasitological examination. Groups I and II were ELISA reactive, whereas Groups III and IV were ELISA nonreactive. Groups II and III were positive for other intestinal parasites. PCR testing scored eight samples as positive from these four groups. Group V represented the S. mansoni -positive group and it included ELISA-reactive samples that were scored positive for S. mansoni by one or more parasitological examinations (6/19 were positive by Kato-Katz method, 9/17 by saline gradient and 10/13 by Helmintex®). PCR scored 13 of these 19 samples as positive for S. mansoni . We conclude that while none of these methods yielded 100% sensitivity, a combination of techniques should be effective for improving the detection of S. mansoni infection in low-endemicity areas.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 4%
Mali 1 2%
France 1 2%
Unknown 44 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 19%
Student > Bachelor 7 15%
Student > Ph. D. Student 7 15%
Student > Doctoral Student 4 8%
Student > Postgraduate 3 6%
Other 7 15%
Unknown 11 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 25%
Medicine and Dentistry 11 23%
Biochemistry, Genetics and Molecular Biology 6 13%
Immunology and Microbiology 4 8%
Psychology 3 6%
Other 1 2%
Unknown 11 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2015.
All research outputs
#2,448,426
of 4,734,919 outputs
Outputs from Memórias do Instituto Oswaldo Cruz
#218
of 455 outputs
Outputs of similar age
#82,044
of 159,074 outputs
Outputs of similar age from Memórias do Instituto Oswaldo Cruz
#12
of 19 outputs
Altmetric has tracked 4,734,919 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 455 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 159,074 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.