Chapter title |
Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants
|
---|---|
Chapter number | 22 |
Book title |
Baculovirus and Insect Cell Expression Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3043-2_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3042-5, 978-1-4939-3043-2
|
Authors |
Hsuan-Chen Wu, Hyung Joon Cha, William E. Bentley, Wu, Hsuan-Chen, Cha, Hyung Joon, Bentley, William E. |
Abstract |
By use of a strategy incorporating the green fluorescent protein (GFP), facile and rapid monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two techniques for simple determination of virus titer in the baculovirus expression system using GFP co-expression and rapid monitoring of Sf-9 insect cell infection using a combination of GFP and the early-to-late (ETL) promoter of the virus vector. Because of its early appearance, GFP, when placed under the control of ETL promoter, will facilitate vector construction, virus isolation, and titer determination. |
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Unknown | 2 | 33% |
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