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Effect of BBX-B8 overexpression on development, body weight, silk protein synthesis and egg diapause of Bombyx mori

Overview of attention for article published in Transgenic Research, March 2016
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Title
Effect of BBX-B8 overexpression on development, body weight, silk protein synthesis and egg diapause of Bombyx mori
Published in
Transgenic Research, March 2016
DOI 10.1007/s11248-016-9947-3
Pubmed ID
Authors

Xiaojian Zheng, Yongchang Gong, Dhiraj Kumar, Fei Chen, Sulan Kuan, Zi Liang, Xiaolong Hu, Guangli Cao, Renyu Xue, Chengliang Gong

Abstract

Bombyxin (BBX) is an insulin-like peptide exists in the silkworm Bombyx mori. Our previous studies on the effects of inhibiting BBX-B8 expression found that BBX-B8 is important for the development of organ, reproduction and trehalose metabolism in the silkworms. In this paper, we investigated the expression profile of the BBX-B8 gene and effect of BBX-B8 overexpression on the development, body weight, silk protein synthesis and egg diapause of B. mori to further understand BBX-B8 functions. BBX-B8 gene expression could be detected in the brains, midguts, anterior silkglands, ovaries, testes, fat bodies, hemolymph, malpighian tubules and embryos by RT-PCR, however it was mainly expressed in the brain. Western blots showed that the change in BBX-B8 expression was not obvious in the brain of 1- to 4-day-old larvae of fifth instar silkworms, but expression increased substantially at 5- to 6-day-old larvae of fifth instar silkworms. Transgenic silkworms overexpressing BBX-B8 were obtained by introducing non-transposon transgenic vector pIZT-B8 containing a BBX-B8 gene driven by Orgyia pseudotsugata nucleopolyhedrovirus IE2 promoter into the genome. Development duration of the transgenic silkworms was delayed by 2.5-3.5 days. Cocoon shell weight of transgenic silkworms was reduced by 4.79 % in females and 7.44 % in males, pupal weight of transgenic silkworms was reduced 6.75 % in females and 13.83 % in males compared to non-transgenic silkworms, and 5.56-14.29 % of transgenic moths laid nondiapausing eggs. All results indicated that BBX-B8 plays an important role in the development, silk protein synthesis and egg diapause of silkworm.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 31%
Researcher 4 31%
Student > Ph. D. Student 3 23%
Unspecified 1 8%
Unknown 1 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 38%
Biochemistry, Genetics and Molecular Biology 4 31%
Unspecified 1 8%
Medicine and Dentistry 1 8%
Unknown 2 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
All research outputs
#20,458,307
of 23,015,156 outputs
Outputs from Transgenic Research
#818
of 895 outputs
Outputs of similar age
#253,047
of 299,563 outputs
Outputs of similar age from Transgenic Research
#24
of 25 outputs
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