Title |
Evidence of G-protein-coupled receptor and substrate transporter heteromerization at a single molecule level
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Published in |
Cellular and Molecular Life Sciences, December 2017
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DOI | 10.1007/s00018-017-2728-1 |
Pubmed ID | |
Authors |
Jana Fischer, Gunnar Kleinau, Claudia Rutz, Denise Zwanziger, Noushafarin Khajavi, Anne Müller, Maren Rehders, Klaudia Brix, Catherine L. Worth, Dagmar Führer, Heiko Krude, Burkhard Wiesner, Ralf Schülein, Heike Biebermann |
Abstract |
G-protein-coupled receptors (GPCRs) can constitute complexes with non-GPCR integral membrane proteins, while such interaction has not been demonstrated at a single molecule level so far. We here investigated the potential interaction between the thyrotropin receptor (TSHR) and the monocarboxylate transporter 8 (MCT8), a member of the major facilitator superfamily (MFS), using fluorescence cross-correlation spectroscopy (FCCS). Both the proteins are expressed endogenously on the basolateral plasma membrane of the thyrocytes and are involved in stimulation of thyroid hormone production and release. Indeed, we demonstrate strong interaction between both the proteins which causes a suppressed activation of Gq/11 by TSH-stimulated TSHR. Thus, we provide not only evidence for a novel interaction between the TSHR and MCT8, but could also prove this interaction on a single molecule level. Moreover, this interaction forces biased signaling at the TSHR. These results are of general interest for both the GPCR and the MFS research fields. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 24% |
Student > Bachelor | 4 | 14% |
Researcher | 4 | 14% |
Student > Master | 2 | 7% |
Professor | 1 | 3% |
Other | 2 | 7% |
Unknown | 9 | 31% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 9 | 31% |
Agricultural and Biological Sciences | 3 | 10% |
Chemistry | 2 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Immunology and Microbiology | 1 | 3% |
Other | 3 | 10% |
Unknown | 10 | 34% |