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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Mechanism of intersubunit ketosynthase–dehydratase interaction in polyketide synthases
|
|---|---|
| Published in |
Nature Chemical Biology, January 2018
|
| DOI | 10.1038/nchembio.2549 |
| Pubmed ID | |
| Authors |
Matthew Jenner, Simone Kosol, Daniel Griffiths, Panward Prasongpholchai, Lucio Manzi, Andrew S Barrow, John E Moses, Neil J Oldham, Józef R Lewandowski, Gregory L Challis |
| Abstract |
Modular polyketide synthases (PKSs) produce numerous structurally complex natural products that have diverse applications in medicine and agriculture. PKSs typically consist of several multienzyme subunits that utilize structurally defined docking domains (DDs) at their N and C termini to ensure correct assembly into functional multiprotein complexes. Here we report a fundamentally different mechanism for subunit assembly in trans-acyltransferase (trans-AT) modular PKSs at the junction between ketosynthase (KS) and dehydratase (DH) domains. This mechanism involves direct interaction of a largely unstructured docking domain (DD) at the C terminus of the KS with the surface of the downstream DH. Acyl transfer assays and mechanism-based crosslinking established that the DD is required for the KS to communicate with the acyl carrier protein appended to the DH. Two distinct regions for binding of the DD to the DH were identified using NMR spectroscopy, carbene footprinting, and mutagenesis, providing a foundation for future elucidation of the molecular basis for interaction specificity. |
X Demographics
The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 8 | 40% |
| United States | 3 | 15% |
| Netherlands | 1 | 5% |
| Brazil | 1 | 5% |
| Canada | 1 | 5% |
| Germany | 1 | 5% |
| Unknown | 5 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 10 | 50% |
| Members of the public | 9 | 45% |
| Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 101 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 101 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 27 | 27% |
| Researcher | 17 | 17% |
| Student > Bachelor | 16 | 16% |
| Student > Master | 10 | 10% |
| Student > Doctoral Student | 2 | 2% |
| Other | 6 | 6% |
| Unknown | 23 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 38 | 38% |
| Biochemistry, Genetics and Molecular Biology | 20 | 20% |
| Agricultural and Biological Sciences | 10 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 4% |
| Chemical Engineering | 1 | <1% |
| Other | 4 | 4% |
| Unknown | 24 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2018.
All research outputs
#2,247,921
of 22,673,450 outputs
Outputs from Nature Chemical Biology
#1,268
of 3,054 outputs
Outputs of similar age
#55,193
of 440,398 outputs
Outputs of similar age from Nature Chemical Biology
#26
of 47 outputs
Altmetric has tracked 22,673,450 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,054 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.3. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 440,398 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.