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Mendeley readers
| Chapter title |
Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants
|
|---|---|
| Chapter number | 14 |
| Book title |
Plant Programmed Cell Death
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7668-3_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7667-6, 978-1-4939-7668-3
|
| Authors |
Qingyu Wu, David Jackson |
| Abstract |
Programmed cell death (PCD) is an essential component of development, biotic and abiotic responses. Hypersensitive response (HR)-associated cell death activated under pathogen attack is one of the most dramatic manifestations of PCD in plants. Signal transduction through mitogen-activated protein kinase (MAPK) cascades, a very conserved signaling pathway across eukaryotes, is a core mediator for HR-associated PCD. Therefore, monitoring MAPK activation enables the mechanisms underlying HR-associated PCD to be elucidated. Here, we describe the use of a phosphorylation-specific MAPK3/6 antibody to monitor the activation of MAPK3/6 during HR-associated PCD. The technique may be adapted for use in other types of PCD. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Other | 2 | 20% |
| Student > Master | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Unknown | 3 | 30% |