Title |
Transcriptional profiling of genes involved in n-hexadecane compounds assimilation in the hydrocarbon degrading Dietzia cinnamea P4 strain
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Published in |
Brazilian Journal of Microbiology, January 2013
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DOI | 10.1590/s1517-83822013000200044 |
Pubmed ID | |
Authors |
Luciano Procópio, Michele de Cassia Pereira e Silva, Jan Dirk van Elsas, Lucy Seldin |
Abstract |
The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator. |
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 24 | 100% |
Demographic breakdown
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Researcher | 4 | 17% |
Student > Ph. D. Student | 3 | 13% |
Student > Bachelor | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Student > Master | 2 | 8% |
Other | 1 | 4% |
Unknown | 10 | 42% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 25% |
Agricultural and Biological Sciences | 4 | 17% |
Chemistry | 2 | 8% |
Neuroscience | 1 | 4% |
Environmental Science | 1 | 4% |
Other | 0 | 0% |
Unknown | 10 | 42% |