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Phenotypic profiling of CD8+ T cells during Plasmodium vivax blood-stage infection

Overview of attention for article published in BMC Infectious Diseases, January 2015
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Title
Phenotypic profiling of CD8+ T cells during Plasmodium vivax blood-stage infection
Published in
BMC Infectious Diseases, January 2015
DOI 10.1186/s12879-015-0762-x
Pubmed ID
Authors

Natália Satchiko Hojo-Souza, Dhelio Batista Pereira, Lívia Silva Araújo Passos, Pedro Henrique Gazzinelli-Guimarães, Mariana Santos Cardoso, Mauro Shugiro Tada, Graziela Maria Zanini, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara, Lilian Lacerda Bueno

Abstract

BackgroundFor a long time, the role of CD8+ T cells in blood-stage malaria was not considered important because erythrocytes do not express major histocompatibility complex (MHC) class I proteins. While recent evidences suggest that CD8+ T cells may play an important role during the erythrocytic phase of infection by eliminating parasites, CD8+ T cells might also contribute to modulate the host response through production of regulatory cytokines. Thus, the role of CD8+ T cells during blood-stage malaria is unclear. Here, we report the phenotypic profiling of CD8+ T cells subsets from patients with uncomplicated symptomatic P. vivax malaria.MethodsBlood samples were collected from 20 Plasmodium vivax-infected individuals and 12 healthy individuals. Immunophenotyping was conducted by flow cytometry. Plasma levels of IFN-¿, TNF-¿ and IL-10 were determined by ELISA/CBA. Unpaired t-test or Mann¿Whitney test was used depending on the data distribution.Results P. vivax-infected subjects had lower percentages and absolute numbers of CD8+CD45RA+ and CD8+CD45RO+ T cells when compared to uninfected individuals (p¿¿¿0.0002). A significantly lower absolute number of circulating CD8+CD45+CCR7+ cells (p¿=¿0.002) was observed in P. vivax-infected individuals indicating that infection reduces the number of central memory T cells. Cytokine expression was significantly reduced in the naïve T cells from infected individuals compared with negative controls, as shown by lower numbers of IFN-¿+ (p¿=¿0.001), TNF-¿+ (p¿<¿0.0001) and IL-10+ (p¿<¿0.0001) CD8+ T cells. Despite the reduction in the number of CD8+ memory T cells producing IFN-¿ (p¿<¿0.0001), P. vivax-infected individuals demonstrated a significant increase in memory CD8+TNF-¿+ (p¿=¿0.016) and CD8+IL-10+ (p¿=¿0.004) cells. Positive correlations were observed between absolute numbers of CD8+IL-10+ and numbers of CD8+IFN-¿+ (p¿<¿0.001) and CD8+TNF-¿+ T cells (p¿¿¿0.0001). Finally, an increase in the plasma levels of TNF-¿ (p¿=¿0.017) and IL-10 (p¿=¿0.006) and a decrease in the IFN-¿ plasma level (p <0.0001) were observed in the P. vivax-infected individuals.Conclusions P. vivax infection reduces the numbers of different subsets of CD8+ T cells, particularly the memory cells, during blood-stage of infection and enhances the number of CD8+ memory T cells expressing IL-10, which positively correlates with the number of cells expressing TNF-¿ and IFN-¿.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 4%
France 1 4%
Brazil 1 4%
Unknown 23 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 27%
Unspecified 4 15%
Student > Master 4 15%
Student > Doctoral Student 3 12%
Student > Ph. D. Student 2 8%
Other 6 23%
Readers by discipline Count As %
Unspecified 7 27%
Agricultural and Biological Sciences 6 23%
Immunology and Microbiology 5 19%
Medicine and Dentistry 4 15%
Nursing and Health Professions 1 4%
Other 3 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2016.
All research outputs
#5,432,580
of 7,216,446 outputs
Outputs from BMC Infectious Diseases
#2,415
of 3,283 outputs
Outputs of similar age
#155,753
of 233,659 outputs
Outputs of similar age from BMC Infectious Diseases
#103
of 151 outputs
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