Title |
Comparative genomic survey of Bacillus cereus sensu stricto isolates from the dairy production chain in Brazil
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Published in |
FEMS Microbiology Letters, December 2017
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DOI | 10.1093/femsle/fnx283 |
Pubmed ID | |
Authors |
Gabriel Augusto Marques Rossi, Higor Oliveira Silva, Carlos Eduardo Gamero Aguilar, Arina Lázaro Rochetti, Ben Pascoe, Guillaume Méric, Evangelos Mourkas, Matthew D Hitchings, Luis Antonio Mathias, Vera Letticie de Azevedo Ruiz, Heidge Fukumasu, Samuel K Sheppard, Ana Maria Centola Vidal |
Abstract |
The genomes of 262 B. cereus isolates were analyzed including 69 isolates sampled from equipment, raw milk and dairy products from Brazil. The population structure of isolates showed strains belonging to known phylogenetic groups II, III, IV, V and VI. Almost all the isolates obtained from dairy products belonged to group III. Investigation of specific alleles revealed high numbers of isolates carrying toxin associated genes including cytK (53.62%), hblA (59.42%), hblC (44.93%), hblD (53.62%), nheA (84.06%), nheB (89.86%), nheC (84.06%) with isolates belonging to groups IV and V having significant higher prevalence of hblACD and group IV of CytK genes. Strains from dairy products had significantly lower prevalence of CytK and hblACD genes compared to isolates from equipment and raw milk/bulk tanks. Genes related to sucrose metabolism were detected at higher frequency in isolates obtained from raw milk compared to strains from equipment and utensils. The population genomic analysis demonstrated the diversity of strains and variability of putative function among B. cereus group isolates in Brazilian dairy production, with large numbers of strains potentially able to cause foodborne illness. This detailed information will contribute to targeted interventions to reduce milk contamination and spoilage associated with B. cereus in Brazil. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 5 | 56% |
Australia | 1 | 11% |
Unknown | 3 | 33% |
Demographic breakdown
Type | Count | As % |
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Scientists | 5 | 56% |
Members of the public | 4 | 44% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 48 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 10 | 21% |
Researcher | 7 | 15% |
Student > Ph. D. Student | 6 | 13% |
Professor > Associate Professor | 4 | 8% |
Student > Bachelor | 3 | 6% |
Other | 5 | 10% |
Unknown | 13 | 27% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 14 | 29% |
Biochemistry, Genetics and Molecular Biology | 5 | 10% |
Immunology and Microbiology | 5 | 10% |
Veterinary Science and Veterinary Medicine | 2 | 4% |
Computer Science | 1 | 2% |
Other | 3 | 6% |
Unknown | 18 | 38% |