| Chapter title |
Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors
|
|---|---|
| Chapter number | 3 |
| Book title |
Sphingosine-1-Phosphate
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/7651_2017_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7412-2, 978-1-4939-7413-9
|
| Authors |
Callihan, Phillip, Alqinyah, Mohammed, Hooks, Shelley B, Phillip Callihan, Mohammed Alqinyah, Shelley B. Hooks |
| Abstract |
Sphingosine-1-phosphate (S1P) and its receptors are important in nervous system development. Reliable in vitro human model systems are needed to further define specific roles for S1P signaling in neural development. We have described S1P-regulated signaling, survival, and differentiation in a human embryonic stem cell-derived neuroepithelial progenitor cell line (hNP1) that expresses functional S1P receptors. These cells can be further differentiated to a neuronal cell type and therefore represent a good model system to study the role of S1P signaling in human neural development. The following sections describe in detail the culture and differentiation of hNP1 cells and two assays to measure S1P signaling in these cells. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 33% |
| Student > Bachelor | 2 | 17% |
| Other | 1 | 8% |
| Professor | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 17% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Neuroscience | 2 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Chemistry | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 33% |