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Glioblastoma

Overview of attention for book
Cover of 'Glioblastoma'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Whole Genome Sequencing-Based Discovery of Structural Variants in Glioblastoma
  3. Altmetric Badge
    Chapter 2 Whole Genome DNA Methylation Analysis of Human Glioblastoma Using Illumina BeadArrays
  4. Altmetric Badge
    Chapter 3 Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
  5. Altmetric Badge
    Chapter 4 Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens
  6. Altmetric Badge
    Chapter 5 Isolation of Glioblastoma Stem Cells with Flow Cytometry
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    Chapter 6 Lentiviral Transduction of Primary Human Glioblastoma Cultures
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    Chapter 7 Selective Targeting of CD133-Expressing Glioblastoma Stem Cells Using Lentiviral Vectors
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    Chapter 8 Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF
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    Chapter 9 Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro
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    Chapter 10 Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography–Tandem Mass Spectrometry
  12. Altmetric Badge
    Chapter 11 Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS)
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    Chapter 12 Single-Cell RNA Sequencing of Glioblastoma Cells
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    Chapter 13 Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures
  15. Altmetric Badge
    Chapter 14 Orthotopic Patient-Derived Glioblastoma Xenografts in Mice
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    Chapter 15 Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice
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    Chapter 16 Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy
  18. Altmetric Badge
    Chapter 17 Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection
  19. Altmetric Badge
    Chapter 18 Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma
  20. Altmetric Badge
    Chapter 19 Modeling Glioma with Human Embryonic Stem Cell-Derived Neural Lineages
Attention for Chapter 3: Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
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Chapter title
Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
Chapter number 3
Book title
Glioblastoma
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7659-1_3
Pubmed ID
Book ISBNs
978-1-4939-7658-4, 978-1-4939-7659-1
Authors

Amit D. Gujar, Diane D. Mao, John B. Finlay, Albert H. Kim

Abstract

This chapter describes a method for isolation, maintenance, and propagation of primary glioblastoma (GBM) cells in adherent monolayer cultures from patient tumor specimens. This method enables the establishment of GBM cultures with stem or progenitor-like cell characteristics, including self-renewal capacity, differentiation along restricted neural lineages, and tumor-initiating potential when orthotopically injected into immunocompromised mice. This experimentally tractable model system is therefore suitable for a wide variety of analyses in vitro as well as in vivo. Key examples of biological analyses that can be performed using these cells are also described.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 30%
Student > Doctoral Student 2 20%
Researcher 2 20%
Student > Bachelor 1 10%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 40%
Chemistry 2 20%
Agricultural and Biological Sciences 1 10%
Neuroscience 1 10%
Unknown 2 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2019.
All research outputs
#17,372,007
of 25,492,047 outputs
Outputs from Methods in molecular biology
#6,036
of 14,224 outputs
Outputs of similar age
#284,448
of 450,224 outputs
Outputs of similar age from Methods in molecular biology
#591
of 1,486 outputs
Altmetric has tracked 25,492,047 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,224 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 450,224 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,486 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.