Chapter title |
Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
|
---|---|
Chapter number | 3 |
Book title |
Glioblastoma
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7659-1_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7658-4, 978-1-4939-7659-1
|
Authors |
Amit D. Gujar, Diane D. Mao, John B. Finlay, Albert H. Kim |
Abstract |
This chapter describes a method for isolation, maintenance, and propagation of primary glioblastoma (GBM) cells in adherent monolayer cultures from patient tumor specimens. This method enables the establishment of GBM cultures with stem or progenitor-like cell characteristics, including self-renewal capacity, differentiation along restricted neural lineages, and tumor-initiating potential when orthotopically injected into immunocompromised mice. This experimentally tractable model system is therefore suitable for a wide variety of analyses in vitro as well as in vivo. Key examples of biological analyses that can be performed using these cells are also described. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 30% |
Student > Doctoral Student | 2 | 20% |
Researcher | 2 | 20% |
Student > Bachelor | 1 | 10% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 40% |
Chemistry | 2 | 20% |
Agricultural and Biological Sciences | 1 | 10% |
Neuroscience | 1 | 10% |
Unknown | 2 | 20% |