Title |
Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin
|
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Published in |
International Journal of Legal Medicine, July 1994
|
DOI | 10.1007/bf01371335 |
Pubmed ID | |
Authors |
Armando Mannucci, Kevin M. Sullivan, Pavel L. Ivanov, Peter Gill |
Abstract |
Gender identification of forensic samples was determined by amplifying a segment of the X-Y homologous gene amelogenin. Using a single pair of primers spanning part of the first intron, 106 bp and 112 bp PCR products were generated from the X and Y homologues respectively, which were then resolved by agarose gel electrophoresis. This test enabled as little as 20 pg of DNA from severely degraded bones to be amplified and typed in a single tube reaction. Furthermore, using dye-labelled primers, it was possible to quantitate, by automated fluorescence detection, the relative yields of X and Y-specific PCR products generated from mixtures of male and female DNA. The versatility of this sex test was further demonstrated by co-amplifying with the HLA-DQA1 Amplitype kit in a combined gender/identity DNA test. |
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