| Title |
Sequence of a cDNA encoding human keratin No 10 selected according to structural homologies of keratins and their tissue-specific expression
|
|---|---|
| Published in |
Molecular Biology Reports, December 1987
|
| DOI | 10.1007/bf00444680 |
| Pubmed ID | |
| Authors |
Michel Y. Darmon, Alix Sémat, Michèle C. Darmon, Marc Vasseur |
| Abstract |
We present here the nucleotide sequence of a 1700 bp-long cDNA encoding human epidermal keratin No. 10 (56.5 kDa). cDNA clones of the acidic keratin family were first isolated from a pBR322 human epidermal cDNA library by hybridization with a probe coding for keratin No. 14. Differential hybridization using total cDNA probes prepared from poly(A)+ RNA extracted either from epidermis (which contains keratin No. 10) and from squamous carcinoma or hepatoma cell lines (which do not express keratin No. 10) made possible the selection of clones potentially coding for keratin No. 10. The 1.7 kb sequence exhibits the characteristic features of an acidic keratin with a constant central rod domain and C-terminal variable structures. Moreover, the sequence shows extensive homologies with the cDNA of murine keratin No. 10. |
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This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2017.
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#8,534,528
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