Title |
Impairment of mitochondrial calcium handling in a mtSOD1 cell culture model of motoneuron disease
|
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Published in |
BMC Neuroscience, June 2009
|
DOI | 10.1186/1471-2202-10-64 |
Pubmed ID | |
Authors |
Manoj Kumar Jaiswal, Wolf-Dieter Zech, Miriam Goos, Christine Leutbecher, Alberto Ferri, Annette Zippelius, Maria Teresa Carrì, Roland Nau, Bernhard U Keller |
Abstract |
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the selective loss of motor neurons (MN) in the brain stem and spinal cord. Intracellular disruptions of cytosolic and mitochondrial calcium have been associated with selective MN degeneration, but the underlying mechanisms are not well understood. The present evidence supports a hypothesis that mitochondria are a target of mutant SOD1-mediated toxicity in familial amyotrophic lateral sclerosis (fALS) and intracellular alterations of cytosolic and mitochondrial calcium might aggravate the course of this neurodegenerative disease. In this study, we used a fluorescence charged cool device (CCD) imaging system to separate and simultaneously monitor cytosolic and mitochondrial calcium concentrations in individual cells in an established cellular model of ALS. |
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