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Development of a lateral flow recombinase polymerase assay for the diagnosis of Schistosoma mansoni infections

Overview of attention for article published in Analytical Biochemistry, April 2018
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  • Good Attention Score compared to outputs of the same age and source (76th percentile)

Mentioned by

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3 tweeters

Citations

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9 Dimensions

Readers on

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26 Mendeley
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Title
Development of a lateral flow recombinase polymerase assay for the diagnosis of Schistosoma mansoni infections
Published in
Analytical Biochemistry, April 2018
DOI 10.1016/j.ab.2018.01.031
Pubmed ID
Authors

Kate Poulton, Bonnie Webster

Abstract

Infection with Schistosoma mansoni causes intestinal schistosomiasis, a major health problem across Africa. The accurate diagnosis of intestinal schistosomiasis is vital to inform surveillance/control programs. Diagnosis mainly relies on microscopic detection of eggs in faecal samples but many factors affect sensitivity. Molecular diagnostics are sensitive and specific but application is limited as necessary infrastructure, financial resources and skilled personnel are often lacking in endemic settings. Recombinase Polymerase Amplification (RPA) is an isothermal DNA amplification/detection technology that is practical in nearly any setting. Here we developed a RPA lateral flow (LF) assay targeting the 28S rDNA region of S. mansoni. The 28S LF-RPA assay's lower limit of detection was 10pg DNA with the lower test parameters permitting sufficient amplification being 6 min and 25°C. Optimal assay parameters were 40-45°C and 10 min with an analytical sensitivity of 102 copies of DNA. Additionally the PCRD3 lateral flow detection cassettes proved more robust and sensitive compared to the Milenia HybriDetect strips. This 28S LF-RPA assay produces quick reproducible results that are easy to interpret, require little infrastructure and is a promising PON test for the field molecular diagnosis of intestinal schistosomiasis.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 23%
Student > Master 6 23%
Other 3 12%
Student > Bachelor 2 8%
Student > Postgraduate 2 8%
Other 4 15%
Unknown 3 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 35%
Engineering 3 12%
Agricultural and Biological Sciences 2 8%
Nursing and Health Professions 1 4%
Business, Management and Accounting 1 4%
Other 4 15%
Unknown 6 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 December 2018.
All research outputs
#8,086,900
of 14,061,361 outputs
Outputs from Analytical Biochemistry
#6,310
of 7,126 outputs
Outputs of similar age
#174,573
of 358,129 outputs
Outputs of similar age from Analytical Biochemistry
#8
of 51 outputs
Altmetric has tracked 14,061,361 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,126 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 358,129 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.