Title |
Using pyrosequencing to shed light on deep mine microbial ecology
|
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Published in |
BMC Genomics, March 2006
|
DOI | 10.1186/1471-2164-7-57 |
Pubmed ID | |
Authors |
Robert A Edwards, Beltran Rodriguez-Brito, Linda Wegley, Matthew Haynes, Mya Breitbart, Dean M Peterson, Martin O Saar, Scott Alexander, E Calvin Alexander, Forest Rohwer |
Abstract |
Contrasting biological, chemical and hydrogeological analyses highlights the fundamental processes that shape different environments. Generating and interpreting the biological sequence data was a costly and time-consuming process in defining an environment. Here we have used pyrosequencing, a rapid and relatively inexpensive sequencing technology, to generate environmental genome sequences from two sites in the Soudan Mine, Minnesota, USA. These sites were adjacent to each other, but differed significantly in chemistry and hydrogeology. |
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Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 23 | 3% |
Brazil | 9 | 1% |
United Kingdom | 7 | 1% |
Spain | 6 | <1% |
India | 5 | <1% |
Germany | 4 | <1% |
France | 3 | <1% |
Portugal | 3 | <1% |
Denmark | 3 | <1% |
Other | 22 | 3% |
Unknown | 575 | 87% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 183 | 28% |
Student > Ph. D. Student | 162 | 25% |
Student > Master | 86 | 13% |
Professor > Associate Professor | 49 | 7% |
Student > Bachelor | 31 | 5% |
Other | 106 | 16% |
Unknown | 43 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 384 | 58% |
Biochemistry, Genetics and Molecular Biology | 59 | 9% |
Environmental Science | 57 | 9% |
Earth and Planetary Sciences | 29 | 4% |
Immunology and Microbiology | 15 | 2% |
Other | 47 | 7% |
Unknown | 69 | 10% |