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Cereal Genomics

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Cover of 'Cereal Genomics'

Table of Contents

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    Book Overview
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    Chapter 1 DNA extraction from vegetative tissue for next-generation sequencing.
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    Chapter 2 DNA extraction from rice endosperm (including a protocol for extraction of DNA from ancient seed samples).
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    Chapter 3 RNA Extraction from Cereal Vegetative Tissue
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    Chapter 4 RNA Extraction from Developing or Mature Wheat Seeds
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    Chapter 5 cDNA Library Preparation
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    Chapter 6 Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants
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    Chapter 7 The Polymerase Chain Reaction (PCR): General Methods
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    Chapter 8 Mutation and Mutation Screening
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    Chapter 9 The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression
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    Chapter 10 Cloning of DNA Fragments: Ligation Reactions in Agarose Gel
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    Chapter 11 Rapid Cloning of Genes and Promoters for Functional Analyses
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    Chapter 12 Genome Walking
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    Chapter 13 Functional Analysis by Protein Biochemistry
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    Chapter 14 Genomic Southern Blot Analysis
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    Chapter 15 Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs
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    Chapter 16 Protein Blotting Protocol for Beginners
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    Chapter 17 Genetic Transformation of Wheat via Particle Bombardment
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    Chapter 18 Sorghum Genetic Transformation by Particle Bombardment
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    Chapter 19 Genetic Transformation of Wheat via Agrobacterium-Mediated DNA Delivery.
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    Chapter 20 A Protocol for High-Throughput Agrobacterium-Mediated Barley Transformation
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    Chapter 21 Agrobacterium-Mediated Transformation: Rice Transformation
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    Chapter 22 Agrobacterium-Mediated Transformation of Maize (Zea mays) Immature Embryos
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    Chapter 23 A Technical Platform for PCR-Based SNP Screening in Cereals and Other Crops
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    Chapter 24 A Method for Discovery of Genome-Wide SNP Between Any Two Genotypes from Whole-Genome Re-sequencing Data
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