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Distinct parts of leukotriene C(4) synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein.

Overview of attention for article published in Biochemical & Biophysical Research Communications, February 2009
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

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5 patents
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1 Wikipedia page

Readers on

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20 Mendeley
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Title
Distinct parts of leukotriene C(4) synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein.
Published in
Biochemical & Biophysical Research Communications, February 2009
DOI 10.1016/j.bbrc.2009.02.074
Pubmed ID
Authors

Tobias Strid, Jesper Svartz, Niclas Franck, Elisabeth Hallin, Björn Ingelsson, Mats Söderström, Sven Hammarström

Abstract

Leukotriene C(4) is a potent inflammatory mediator formed from arachidonic acid and glutathione. 5-Lipoxygenase (5-LO), 5-lipoxygenase activating protein (FLAP) and leukotriene C(4) synthase (LTC(4)S) participate in its biosynthesis. We report evidence that LTC(4)S interacts in vitro with both FLAP and 5-LO and that these interactions involve distinct parts of LTC(4)S. FLAP bound to the N-terminal part/first hydrophobic region of LTC(4)S. This part did not bind 5-LO which bound to the second hydrophilic loop of LTC(4)S. Fluorescent FLAP- and LTC(4)S-fusion proteins co-localized at the nuclear envelope. Furthermore, GFP-FLAP and GFP-LTC(4)S co-localized with a fluorescent ER marker. In resting HEK293/T or COS-7 cells GFP-5-LO was found mainly in the nuclear matrix. Upon stimulation with calcium ionophore, GFP-5-LO translocated to the nuclear envelope allowing it to interact with FLAP and LTC(4)S. Direct interaction of 5-LO and LTC(4)S in ionophore-stimulated (but not un-stimulated) cells was demonstrated by BRET using GFP-5-LO and Rluc-LTC(4)S.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 2 10%
United Kingdom 1 5%
United States 1 5%
Unknown 16 80%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 40%
Student > Ph. D. Student 5 25%
Student > Bachelor 2 10%
Professor > Associate Professor 2 10%
Professor 1 5%
Other 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 55%
Biochemistry, Genetics and Molecular Biology 3 15%
Chemistry 3 15%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Unspecified 1 5%
Other 1 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2016.
All research outputs
#1,184,729
of 9,957,452 outputs
Outputs from Biochemical & Biophysical Research Communications
#231
of 12,608 outputs
Outputs of similar age
#36,836
of 249,061 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#7
of 198 outputs
Altmetric has tracked 9,957,452 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,608 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,061 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 198 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.