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Distinct parts of leukotriene C4 synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein

Overview of attention for article published in Biochemical & Biophysical Research Communications, April 2009
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Mentioned by

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19 patents
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1 Wikipedia page

Citations

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15 Dimensions

Readers on

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20 Mendeley
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Title
Distinct parts of leukotriene C4 synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein
Published in
Biochemical & Biophysical Research Communications, April 2009
DOI 10.1016/j.bbrc.2009.02.074
Pubmed ID
Authors

Tobias Strid, Jesper Svartz, Niclas Franck, Elisabeth Hallin, Björn Ingelsson, Mats Söderström, Sven Hammarström

Abstract

Leukotriene C(4) is a potent inflammatory mediator formed from arachidonic acid and glutathione. 5-Lipoxygenase (5-LO), 5-lipoxygenase activating protein (FLAP) and leukotriene C(4) synthase (LTC(4)S) participate in its biosynthesis. We report evidence that LTC(4)S interacts in vitro with both FLAP and 5-LO and that these interactions involve distinct parts of LTC(4)S. FLAP bound to the N-terminal part/first hydrophobic region of LTC(4)S. This part did not bind 5-LO which bound to the second hydrophilic loop of LTC(4)S. Fluorescent FLAP- and LTC(4)S-fusion proteins co-localized at the nuclear envelope. Furthermore, GFP-FLAP and GFP-LTC(4)S co-localized with a fluorescent ER marker. In resting HEK293/T or COS-7 cells GFP-5-LO was found mainly in the nuclear matrix. Upon stimulation with calcium ionophore, GFP-5-LO translocated to the nuclear envelope allowing it to interact with FLAP and LTC(4)S. Direct interaction of 5-LO and LTC(4)S in ionophore-stimulated (but not un-stimulated) cells was demonstrated by BRET using GFP-5-LO and Rluc-LTC(4)S.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 2 10%
United States 1 5%
United Kingdom 1 5%
Unknown 16 80%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 40%
Student > Ph. D. Student 5 25%
Professor > Associate Professor 2 10%
Student > Bachelor 2 10%
Student > Doctoral Student 1 5%
Other 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 55%
Chemistry 3 15%
Biochemistry, Genetics and Molecular Biology 3 15%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Unspecified 1 5%
Other 1 5%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2018.
All research outputs
#1,010,468
of 11,983,631 outputs
Outputs from Biochemical & Biophysical Research Communications
#152
of 20,387 outputs
Outputs of similar age
#29,632
of 270,013 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#4
of 245 outputs
Altmetric has tracked 11,983,631 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 20,387 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 270,013 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 245 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.