Cellular Heterogeneity
Humana Press, New York, NY
Chapter title |
X-Ray Fluorescence-Detected Flow Cytometry
|
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Chapter number | 6 |
Book title |
Cellular Heterogeneity
|
Published by |
Humana Press, New York, NY, February 2018
|
DOI | 10.1007/978-1-4939-7680-5_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7679-9, 978-1-4939-7680-5
|
Authors |
Andrew M. Crawford, James E. Penner-Hahn |
Abstract |
X-ray fluorescence-detected flow cytometry can enable the detection and characterization of ultra-trace, trace, and bulk elemental content at the cellular level using synchrotron-induced x-ray emission from fully aquated actively respiring cells. Although very much still a technique in development, this technique has been used to characterize cell-to-cell elemental variability in bovine red blood cells, Saccharomyces cerevisiae, and NIH3T3 mouse fibroblasts. Herein we describe the experimental setup and the key methodological aspects of data collection and processing. |
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