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Attenuation of estrogen receptor  -mediated transcription through estrogen-stimulated recruitment of a negative elongation factor

Overview of attention for article published in Genes & Development, September 2004
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

patent
3 patents
wikipedia
2 Wikipedia pages

Citations

dimensions_citation
85 Dimensions

Readers on

mendeley
54 Mendeley
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Title
Attenuation of estrogen receptor  -mediated transcription through estrogen-stimulated recruitment of a negative elongation factor
Published in
Genes & Development, September 2004
DOI 10.1101/gad.1214104
Pubmed ID
Authors

S. E. Aiyar

Abstract

Estrogen receptor alpha (ERalpha) signaling is paramount for normal mammary gland development and function and the repression of breast cancer. ERalpha function in gene regulation is mediated by a number of coactivators and corepressors, most of which are known to modify chromatin structure and/or influence the assembly of the regulatory complexes at the level of transcription initiation. Here we describe a novel mechanism of attenuating the ERalpha activity. We show that cofactor of BRCA1 (COBRA1), an integral subunit of the human negative elongation factor (NELF), directly binds to ERalpha and represses ERalpha-mediated transcription. Reduction of the endogenous NELF proteins in breast cancer cells using small interfering RNA results in elevated ERalpha-mediated transcription and enhanced cell proliferation. Chromatin immunoprecipitation reveals that recruitment of COBRA1 and the other NELF subunits to endogenous ERalpha-responsive promoters is greatly stimulated upon estrogen treatment. Interestingly, COBRA1 does not affect the estrogen-dependent assembly of transcription regulatory complexes at the ERalpha-regulated promoters. Rather, it causes RNA polymerase II (RNAPII) to pause at the promoter-proximal region, which is consistent with its in vitro biochemical activity. Therefore, our in vivo work defines the first corepressor of nuclear receptors that modulates ERalpha-dependent gene expression by stalling RNAPII. We suggest that this new level of regulation may be important to control the duration and magnitude of a rapid and reversible hormonal response.

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 2 4%
France 1 2%
Switzerland 1 2%
United States 1 2%
Unknown 49 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 26%
Researcher 10 19%
Student > Master 7 13%
Professor 4 7%
Professor > Associate Professor 4 7%
Other 15 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 52%
Biochemistry, Genetics and Molecular Biology 16 30%
Unspecified 4 7%
Medicine and Dentistry 4 7%
Neuroscience 2 4%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2016.
All research outputs
#1,243,658
of 11,316,164 outputs
Outputs from Genes & Development
#551
of 4,871 outputs
Outputs of similar age
#37,155
of 250,924 outputs
Outputs of similar age from Genes & Development
#13
of 50 outputs
Altmetric has tracked 11,316,164 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,871 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,924 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 50 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.