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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Expression and characterization of glutamate decarboxylase from Lactobacillus brevis HYE1 isolated from kimchi
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, March 2018
|
| DOI | 10.1007/s11274-018-2427-6 |
| Pubmed ID | |
| Authors |
Hee Seon Lim, Dong-Ho Seo, In-Tae Cha, Hyunjin Lee, Young-Do Nam, Myung-Ji Seo |
| Abstract |
A putative gene (gadlbhye1) encoding glutamate decarboxylase (GAD) was cloned from Lactobacillus brevis HYE1 isolated from kimchi, a traditional Korean fermented vegetable. The amino acid sequences of GADLbHYE1 showed 48% homology with the GadA family and 99% identity with the GadB family from L. brevis. The cloned GADLbHYE1 was functionally expressed in Escherichia coli using inducible expression vectors. The expressed recombinant GADLbHYE1 was successfully purified by Ni-NTA affinity chromatography, and had a molecular mass of 54 kDa with optimal hydrolysis activity at 55 °C and pH 4.0. Its thermal stability was determined to be higher than that of other GADs from L. brevis, based on its melting temperature (75.18 °C). Kinetic parameters including Kmand Vmaxvalues for GADLbHYE1 were 4.99 mmol/L and 0.224 mmol/L/min, respectively. In addition, the production of gamma-aminobutyric acid in E. coli BL21 harboring gadlbhye1/pET28a was increased by adding pyridoxine as a cheaper coenzyme. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 18% |
| Professor | 5 | 15% |
| Student > Bachelor | 3 | 9% |
| Researcher | 2 | 6% |
| Student > Postgraduate | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 12 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 24% |
| Biochemistry, Genetics and Molecular Biology | 4 | 12% |
| Engineering | 3 | 9% |
| Unspecified | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 15 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 March 2018.
All research outputs
#14,759,948
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#852
of 1,757 outputs
Outputs of similar age
#185,611
of 334,643 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#12
of 25 outputs
Altmetric has tracked 23,911,072 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,757 research outputs from this source. They receive a mean Attention Score of 2.9. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,643 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.