| Chapter title |
Sex identification of ancient DNA samples using a microfluidic device.
|
|---|---|
| Chapter number | 8 |
| Book title |
Microchip Capillary Electrophoresis Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2353-3_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2352-6, 978-1-4939-2353-3
|
| Authors |
Kirsty J Shaw, Keri A Brown, Terence A Brown, Stephen J Haswell, Kirsty J. Shaw, Keri A. Brown, Terence A. Brown, Stephen J. Haswell |
| Editors |
Ann Van Schepdael |
| Abstract |
Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Streptavidin-coated superparamagnetic particles are used to isolate the labeled DNA prior to amplification of the Amelogenin sex marker. |
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