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Mendeley readers
Attention Score in Context
| Title |
Isolation and immunocytochemical location of the nitrite-oxidizing system in Nitrospira moscoviensis
|
|---|---|
| Published in |
Archives of Microbiology, February 1998
|
| DOI | 10.1007/s002030050565 |
| Pubmed ID | |
| Authors |
E. Spieck, Silke Ehrich, Jens Aamand, Eberhard Bock |
| Abstract |
A membrane-associated nitrite-oxidizing system of Nitrospira moscoviensis was isolated from heat-treated membranes. The four major proteins of the enzyme fraction had apparent molecular masses of 130, 62, 46, and 29 kDa, respectively. The nitrite-oxidizing activity was dependent on the presence of molybdenum. In contrast to the nitrite oxidoreductase of Nitrobacter hamburgensis X14, the activity of the nitrite-oxidizing system of Ns. moscoviensis increased when solubilized by heat treatment. Electron microscopy of the purified enzyme revealed uniform particles with a size of approximately 7 x 9 nm. SDS-immunoblotting analysis of crude extracts showed that the monoclonal antibodies Hyb 153-3, which recognize the beta-subunit of the nitrite oxidoreductase from Nitrobacter, reacted with a protein of 50 kDa in Ns. moscoviensis. This protein corresponded to the protein of 46 kDa of the purified enzyme and contained a b-type cytochrome. Using electron microscopic immunocytochemistry and the monoclonal antibodies Hyb 153-3, the nitrite-oxidizing system of Ns. moscoviensis was shown to be located in the periplasmic space. Here a periodic arrangement of membrane-associated particles was found on the outside of the cytoplasmic membrane in the form of a hexagonal pattern. It is supposed that these particles represent the nitrite-oxidizing system in Nitrospira. |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| Unknown | 72 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 34% |
| Researcher | 12 | 16% |
| Student > Bachelor | 10 | 14% |
| Student > Master | 10 | 14% |
| Professor | 5 | 7% |
| Other | 7 | 9% |
| Unknown | 5 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 29 | 39% |
| Environmental Science | 14 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 11% |
| Earth and Planetary Sciences | 6 | 8% |
| Engineering | 3 | 4% |
| Other | 5 | 7% |
| Unknown | 9 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2016.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Archives of Microbiology
#641
of 3,119 outputs
Outputs of similar age
#19,961
of 95,114 outputs
Outputs of similar age from Archives of Microbiology
#2
of 7 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,119 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 95,114 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.