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Attention Score in Context
| Title |
Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 °C for 96 hours
|
|---|---|
| Published in |
Reproductive Biology and Endocrinology, March 2018
|
| DOI | 10.1186/s12958-018-0339-9 |
| Pubmed ID | |
| Authors |
Laura Falchi, Grazia Galleri, Gian Mario Dore, Maria Teresa Zedda, Salvatore Pau, Luisa Bogliolo, Federica Ariu, Alessandra Pinna, Stefano Nieddu, Plinio Innocenzi, Sergio Ledda |
| Abstract |
Cerium oxide nanoparticles (CeO2NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO2NPs for 96 h on the main structural and kinematic parameters of spermatozoa. The ejaculates of 5 Sarda rams were collected, pooled and diluted in a soybean lecithin extender. Samples were exposed to increasing doses of CeO2NPs (0, 44 and 220 μg/mL) and stored at 4 °C for 96 h. Analyses of kinematic parameters (computer assisted sperm analysis, CASA), integrity of membranes (PI/PSA staining), ROS production (H2DCFDA staining) and DNA damage (sperm chromatin structure assay with acridine orange, SCSA) were performed every 24 h (0, 24, 48, 72 and 96 h of incubation). The experiment was carried out in 6 replicates. Data were analysed by repeated measures ANOVA with Bonferroni's as post hoc test. When the assumption of normality was not met (ROS), non-parametric Kruskal-Wallis rank test was carried out. Exposure of ram spermatozoa to increasing doses of CeO2NPs had a beneficial effect on the main motility parameters from 48 h of incubation onward. Velocity of sperm cells was enhanced in the groups exposed to CeO2NPs compared to the control. Incubation with NPs had beneficial effects on the integrity of plasma membranes of spermatozoa, with higher percentage of damaged cells in the control group compared to the exposed ones. Production of ROS was not affected by exposure to NPs and its levels rose at 96 h of incubation. The integrity of DNA remained stable throughout the 96 h of storage regardless of co-incubation with NPs. We reported beneficial effects of CeO2NPs on kinematic and morphologic parameters of ram semen, such as motility and membrane integrity following 96 h of exposure. Furthermore, we also proved no genotoxic effects of CeO2NPs. These effects could not be related to an antioxidant activity of CeO2NPs, since ROS levels in exposed cells were similar to those of unexposed ones. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 58 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 14% |
| Student > Doctoral Student | 7 | 12% |
| Researcher | 7 | 12% |
| Lecturer | 5 | 9% |
| Student > Ph. D. Student | 4 | 7% |
| Other | 4 | 7% |
| Unknown | 23 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 7 | 12% |
| Agricultural and Biological Sciences | 7 | 12% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Engineering | 3 | 5% |
| Environmental Science | 2 | 3% |
| Other | 8 | 14% |
| Unknown | 26 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 March 2018.
All research outputs
#18,590,133
of 23,026,672 outputs
Outputs from Reproductive Biology and Endocrinology
#676
of 987 outputs
Outputs of similar age
#258,064
of 331,979 outputs
Outputs of similar age from Reproductive Biology and Endocrinology
#15
of 19 outputs
Altmetric has tracked 23,026,672 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 987 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 10.0. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,979 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.