Chapter title |
Targeted Gene Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases
|
---|---|
Chapter number | 25 |
Book title |
Engineering and Application of Pluripotent Stem Cells
|
Published in |
Advances in biochemical engineering biotechnology, January 2017
|
DOI | 10.1007/10_2017_25 |
Pubmed ID | |
Book ISBNs |
978-3-31-973590-0, 978-3-31-973591-7
|
Authors |
Sylvia Merkert, Ulrich Martin |
Abstract |
Introduction of induced pluripotent stem cell (iPSC) technology and site-directed nucleases brought a major breakthrough in the development of regenerative therapies and biomedical research. With the advancement of ZFNs, TALENs, and the CRISPR/Cas9 technology, straightforward and precise manipulation of the genome of human pluripotent stem cells (PSC) became possible, allowing relatively easy and fast generation of gene knockouts, integration of transgenes, or even introduction of single nucleotide changes for correction or introduction of disease-specific mutations. We review current applications of site-specific nucleases in human PSCs and focus on trends and challenges for efficient gene editing and improvement of targeting strategies. Graphical Abstract. |
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Unknown | 2 | 100% |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 11 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 36% |
Student > Doctoral Student | 1 | 9% |
Professor | 1 | 9% |
Student > Bachelor | 1 | 9% |
Student > Master | 1 | 9% |
Other | 1 | 9% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Medicine and Dentistry | 2 | 18% |
Sports and Recreations | 1 | 9% |
Neuroscience | 1 | 9% |
Materials Science | 1 | 9% |
Other | 0 | 0% |
Unknown | 3 | 27% |