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Effects of growth/differentiation factor 5 on the survival and morphology of embryonic rat midbrain dopaminergic neurones in vitro

Overview of attention for article published in Journal of Neurocytology, September 2004
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#5 of 138)
  • Good Attention Score compared to outputs of the same age (79th percentile)

Mentioned by

patent
5 patents
wikipedia
2 Wikipedia pages

Citations

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43 Dimensions

Readers on

mendeley
24 Mendeley
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1 CiteULike
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Title
Effects of growth/differentiation factor 5 on the survival and morphology of embryonic rat midbrain dopaminergic neurones in vitro
Published in
Journal of Neurocytology, September 2004
DOI 10.1007/s11068-004-0511-y
Pubmed ID
Authors

Gerard W. O’Keeffe, Peter Dockery, Aideen M. Sullivan

Abstract

Growth/differentiation factor 5 (GDF5) is a member of the transforming growth factor-beta superfamily that is expressed in the developing CNS, including the ventral mesencephalon (VM). GDF5 has been shown to increase the survival of dopaminergic neurones in animal models of Parkinson's disease. This study was aimed at characterising the effects of GDF5 on dopaminergic neurones in vitro. Treatment with GDF5 induced a three-fold increase in the number of dopaminergic neurones in embryonic day 14 rat VM cultures after six days in vitro. A significant increase was also observed in the numbers of astrocytes in GDF5-treated cultures. GDF5 treatment also had significant effects on the morphology of dopaminergic neurones in these cultures; total neurite length, number of branch points and somal area were all significantly increased after six days in vitro. Analysis of neurite length and numbers of branch points at each level of the neuritic field revealed that the most pronounced effects of GDF5 were on the secondary and tertiary levels of the neuritic field. The specific type I receptor for GDF5, bone morphogenetic protein receptor (BMPR)-Ib, was found to be strongly expressed in freshly-dissected E14 VM tissue, but its expression was lost with increasing time in culture. Accordingly, treatment with GDF5 for 24 h from the time of plating induced increases in the numbers of dopaminergic neurones, while treatment with GDF5 for 24 h after six days in vitro did not. This study shows that GDF5 can promote both the survival and morphological differentiation of VM dopaminergic neurones in vitro, lending support to its potential as a candidate dopaminergic neurotrophin for use in the treatment of Parkinson's disease.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 4%
Unknown 23 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 25%
Student > Bachelor 5 21%
Professor 3 13%
Researcher 2 8%
Student > Doctoral Student 1 4%
Other 2 8%
Unknown 5 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 42%
Medicine and Dentistry 3 13%
Engineering 2 8%
Neuroscience 2 8%
Chemistry 1 4%
Other 1 4%
Unknown 5 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 April 2019.
All research outputs
#1,767,965
of 10,895,663 outputs
Outputs from Journal of Neurocytology
#5
of 138 outputs
Outputs of similar age
#50,162
of 250,058 outputs
Outputs of similar age from Journal of Neurocytology
#1
of 1 outputs
Altmetric has tracked 10,895,663 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 138 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,058 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them