Title |
Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA
|
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Published in |
Brazilian Journal of Microbiology, February 2017
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DOI | 10.1016/j.bjm.2016.11.007 |
Pubmed ID | |
Authors |
Ekaterina Alexandrovna Durymanova Ono, Sueli Akemi Taniwaki, Paulo Brandão |
Abstract |
The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1log decrease in virus titter and 5.16-fold reduction in P mRNA, 24h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce partial protection, but with no significant difference when compared to non-treated mice. These results indicate that, despite the need for improvement for in vivo applications, P mRNA might be a target for an RNAi-based treatment for rabies. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Australia | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 5 | 25% |
Researcher | 4 | 20% |
Student > Ph. D. Student | 2 | 10% |
Student > Bachelor | 1 | 5% |
Other | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 35% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 20% |
Computer Science | 2 | 10% |
Agricultural and Biological Sciences | 2 | 10% |
Environmental Science | 1 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Other | 3 | 15% |
Unknown | 7 | 35% |