| Chapter title |
A Complete Guide to Using the Endothelin-1 Model of Stroke in Conscious Rats for Acute and Long-Term Recovery Studies
|
|---|---|
| Chapter number | 10 |
| Book title |
Traumatic and Ischemic Injury
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7526-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7524-2, 978-1-4939-7526-6
|
| Authors |
Hima C. S. Abeysinghe, Carli L. Roulston, Abeysinghe, Hima C. S., Roulston, Carli L. |
| Abstract |
Multiple methods exist to model permanent and transient ischemia under anesthesia in animals, however most human strokes occur while conscious. The use of endothelin-1 as a vasoconstrictor applied to the perivascular surface of the middle cerebral artery is one of the only methods for inducing stroke in conscious animals. Here, we describe standard operating procedures for stereotaxic placement of an ET-1 guide probe above the middle cerebral artery, induction of stroke in conscious rats, predictive outcome scoring during stroke, and neurological behavioral tests that we use to monitor transient and continuing deficits. The inclusion of long term neurological assessment is of particular importance when taking into consideration the effects of stroke on brain remodeling. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 19% |
| Student > Ph. D. Student | 5 | 19% |
| Lecturer | 3 | 11% |
| Student > Bachelor | 2 | 7% |
| Student > Doctoral Student | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 9 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 19% |
| Neuroscience | 5 | 19% |
| Medicine and Dentistry | 2 | 7% |
| Agricultural and Biological Sciences | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 10 | 37% |