https://t.co/tSC06wZpwY "The human genome is generally organized into stable chromosomes, and only tumor cells are known to accumulate kilobase (kb)-sized extrachromosomal circular DNA elements (eccDNAs)."
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🧬How are eccDNAs purified and sequenced? Usually, their circularity is exploited: While linear DNA is cut by exonucleases, circular DNA is enriched by rolling-cycle amplification. Check out these studies in🌱and🏃: 🌱https://t.co/tjiHJD6ZcV 🏃https://t.co
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@erikgarrison @MikeEberle @ZaminIqbal @pathogenomenick @DNAmlin @ewanbirney This is not PCR but it might help. I work on extrachromosomal DNA circles. Since our input DNA is scarce, we amplify the circles with a phi29 polymerase. I have observe circles at